stem cell assays / edited by Mohan C. Vemuri. — Totowa : Humana Press, c2007. – (58.17/M592/v.407) |
Contents
Contents
Preface v
Contributors xi
1 Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions
2 Feeder-Layer Free Culture System for Human Embryonic Stem Cells
3 Digital Imaging of Stem Cells by Electron Microscopy
4 A Controlled-Cooling Protocol for Cryopreservation of Human and Non-Human Primate Embryonic Stem Cells
5 Cell Surface Markers in Human Embryonic Stem Cells
6 Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells
7 Analytical Methods for Cancer Stem Cells
8 Micro RNA Profiling: An Easy and Rapid Method to Screen and Characterize Stem Cell Populations
9 Gene Transfer Via Nucleofection Into Adult and Embryonic Stem Cells
10 RNAi Knockdown of Transcription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem Cells
11 StemBase: A Resource for the Analysis of Stem Ceil Gene Expression Data
12 Isolation of Stem Cells from Human Umbilical Cord Blood
13 Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free Conditions
14 Hematopoietic Colony-Forming Cell Assays
15 Assays for AIIoreactive Responses by PCR
16 Immune Properties of Mesenchymal Stem Cells
17 Clinical Grade Expansion of Human Bone Marrow Mesenchymal Stem Cells
18 Adenoviral Transduction of Mesenchymal Stem Cells
19 Directed Differentiation of Human Embryonic Stem Cells to Dendritic Cells
20 Insulin-Producing Cells from Embryonic Stem Cells Experimental Considerations
21 Efficient Generation of Dopamine Neurons from Human Embryonic Stem Cells
22 Isolation of Oligodendroglial Cells from Cultured Neural Stem/Progen itors
23 Differentiation of Human Embryonic Stem Cells Toward the Chondrogenic Lineage
24 Cartilage Tissue Engineering: Directed Differentiation of Embryonic Stem Cells in Three-Dimensional Hydrogel Culture