Affinity chromatography : method and protocols / edited by Michael Zachariou. — 2nd ed. — Totowa : Humana Press, c2008. – (58.17/M592/v.421)

Contents

    Contents
    
    Preface
    Contributors
    1. Affinity Chromatography: History， Perspectives， Limitations and Prospects
    PART I: VARIOUS MODES OF AFFINITY CHROMATOGRAPHY
    2. Immobilized Metal Ion Affinity Chromatography of Native Proteins 25
    3. Affinity Precipitation of Proteins Using Metal Chelates 37
    4. Immunoaffinity Chromatography 53
    5. Dye Ligand Chromatography
    6. Purification of Proteins Using Displacement Chromatography 71
    PART I1: AFFINITY CHROMATOGRAPHY USING PURIFICATION TAGS
    7. Rationally Designed Ligands for Use in Affinity Chromatography: An Artificial Protein L 93
    8. Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography
    9. Preparation， Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein 125
    10. Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins 137
    11. Methods for the Purification of HQ-Tagged Proteins 151
    12. Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis Refolding Methods 169
    13. Methods for Detection of Protein-Protein and Protein-DNA Interactions Using HaloTagTM 191
    14. Site-Specific Cleavage of Fusion Proteins 211
    15. The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags 229
    PART II1: VARIOUS APPLICATIONS OF AFFINITY CHROMATOG RAPHY
    16. Affinity Processing of Ceil-Containing Feeds Using Monolithic Macroporous Hydrogels， Cryogels
    17. Monolithic Bioreactors for Macromolecules
    18. Plasmid DNA Purification Via she Use of a Dual Affinity Protein
    19. Affinity Chromatography of Phosphorylated Proteins
    20. Protein Separation Using Immobilized Phospholipid Chromatography
    21. Analysis of Proteins in Solution Using Affinity Capillary Electrophoresis