Methods in microbiology. v. 36, Yeast gene analysis / edited by Ian Stansfield and Michael JR Stark. — 2nd ed. — Amsterdam : Elsevier, c2007.—(58.605/N856/v.36) |
Contents
Contents
Series Advisors.
Contributors
Preface
1. Introduction to Functional Analysis in Yeast 1
2. Yeast Genetics and Strain Construction 23
3. Yeast Transformation 45
4. A Guided Tour to PCR-based Genomic Manipulations of S. cerevisiae (PCR-targeting) 55
5. Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles 79
6. Yeast Hybrid Approaches 103
7. Array-Based Yeast Two-Hybrid Screening for Protein-Protein Interactions 139
8. Reporter Genes and Their Uses in Studying Yeast Gene Expression 165
9. Transcript Analysis: A Microarray Approach 189
10. GFP-based Microscopic Approaches for Whole Chromosome Analysis in Yeasts
11. Immunological Methods 241
12. Measuring the Proximity of Proteins in Living Cells by Fluorescence Resonance Energy Transfer between CFP and YFP 269
13. Identification, Characterization, and Phenotypic Analysis of Covalently Linked Cell Wall Proteins 281
14. Yeast Protein Microarrays 303
15. Smart Genetic Screens 331
16. High-Throughput Strain Construction and Systematic Synthetic Lethal Screening in Saccharomyces cerevisiae 369
17. Chemical Genomic Tools for Understanding Gene Function and Drug Action 387
18. RNA Gene Analysis
19. Analysis of Gene Function of Mitochondria 445
20. Yeast Prions and Their Analysis In Vivo 491
21. Metabolic Control in the Eukaryotic Cell, a Systems Biology Perspective 527
22. Phylogenetic Footprinting 551
23. Saccharomyces cerevisiae as a Tool for Human Gene Function Discovery 577
24. Bioinformatic Prediction of Yeast Gene Function 597
25. Yeast Genetic Strain and Plasmid Collections 629
26. Yeast Gene Analysis: The Remaining Challenges 667