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Methods in microbiology. v. 36, Yeast gene analysis / edited by Ian Stansfield and Michael JR Stark. — 2nd ed. — Amsterdam : Elsevier, c2007.—(58.605/N856/v.36)

Contents

    Contents
    
    Series Advisors.
    Contributors
    Preface
    1. Introduction to Functional Analysis in Yeast 1
    2. Yeast Genetics and Strain Construction 23
    3. Yeast Transformation 45
    4. A Guided Tour to PCR-based Genomic Manipulations of S. cerevisiae (PCR-targeting) 55
    5. Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles 79
    6. Yeast Hybrid Approaches 103
    7. Array-Based Yeast Two-Hybrid Screening for Protein-Protein Interactions 139
    8. Reporter Genes and Their Uses in Studying Yeast Gene Expression 165
    9. Transcript Analysis: A Microarray Approach 189
    10. GFP-based Microscopic Approaches for Whole Chromosome Analysis in Yeasts
    11. Immunological Methods 241
    12. Measuring the Proximity of Proteins in Living Cells by Fluorescence Resonance Energy Transfer between CFP and YFP 269
    13. Identification, Characterization, and Phenotypic Analysis of Covalently Linked Cell Wall Proteins 281
    14. Yeast Protein Microarrays 303
    15. Smart Genetic Screens 331
    16. High-Throughput Strain Construction and Systematic Synthetic Lethal Screening in Saccharomyces cerevisiae 369
    17. Chemical Genomic Tools for Understanding Gene Function and Drug Action 387
    18. RNA Gene Analysis
    19. Analysis of Gene Function of Mitochondria 445
    20. Yeast Prions and Their Analysis In Vivo 491
    21. Metabolic Control in the Eukaryotic Cell, a Systems Biology Perspective 527
    22. Phylogenetic Footprinting 551
    23. Saccharomyces cerevisiae as a Tool for Human Gene Function Discovery 577
    24. Bioinformatic Prediction of Yeast Gene Function 597
    25. Yeast Genetic Strain and Plasmid Collections 629
    26. Yeast Gene Analysis: The Remaining Challenges 667