Electron microscopy : methods and protocols / edited by John Kuo. — 2nd ed. — Totowa : Humana Press, c2007.—(58.17/M592/v.369) |
Contents
Contents
Preface
Acknowledgments
1 Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells
2 Processing Biological Tissues for Ultrastructural Study
3 Processing Plant Tissues for Ultrastructural Study
4 Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy
5 Ultramicrotomy for Biological Electron Microscopy
6 Poststaining Grids for Transmission Electron Microscopy: Conventional and Alternative Protocols
7 Negative Staining of Thinly Spread Biological Samples
8 Recent Advances in High-Pressure Freezing: Equipment- and Specimen-Loading Methods
9 Cryoultramicrotomy: Cryoelectron Microscopy of Vitreous Sections
10 Cell-Free Extract Systems and the Cytoskeleton: Preparation of Biochemical Experiments for Transmission Electron Microscopy
11 Electron Microscopy In Situ Hybridization: Tracking of DNA and RNA Sequences at High Resolution
12 Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections
13 Cryosectioning Fixed and Cryoprotected Biological Material for Immunocytochemistry
14 Fluorescence-Integrated Transmission Electron Microscopy Images: Integrating Fluorescence Microscopy With Transmission Electron Microscopy
15 Quantitative Immunoelectron Microscopy: Alternative Ways of Assessing Subcellular Patterns of Gold Labeling
16 Electron Crystallography of Membrane Proteins
17 Cryoelectron Microscopy of Icosahedral Virus Particles
18 Three-Dimensional Reconstruction of Chromosomes Using Electron Tomography
19 Whole-Mount Immunoelectron Tomography of Chromosomes and Cells
20 Three-Dimensional Cryotransmission Electron Microscopy of Cells and Organelles
21 Electron Energy-Loss Spectroscopy as a Tool for Elemental Analysis in Biological Specimens
22 Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specimens
23 Variable Pressure and Environmental Scanning Electron Microscopy: Imaging of Biological Samples
24 Cryoplaning Technique for Visualizing the Distribution of Water in Woody Tissues by Cryoscanning Electron Microscopy
25 X-Ray Microanalysis in the Scanning Electron Microscope
26 Botanical X-Ray Microanalysis in Cryoscanning Electron Microscopy
27 Static Secondary Ion Mass Spectrometry for Biological and Biomedical Research
28 Using SIMS and MIMS in Biological Materials: Application to Higher Plants