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Biological aging : methods and protocols / edited by Trygve O. Tollefsbol. — Totowa : Humana Press, c2007.—(58.17/M592/v.371) |
Contents
Contents
Preface
Contributors
1 Techniques for Analysis of Biological Aging
2 Aging Cell Culture: Methods and Observations
3 Methods to Detect Biomarkers of Cellular Senescence: The Senescence-Associated β-Galactosidase
4 Methods of Cell Sorting of Young and Senescent Cells
5 Telomere Length Analysis
6 A Method to Detect DNA Methyltransferase I Gene Transcription In Vitro in Aging Systems
7 A Method to Study the Expression of DNA Methyltransferase in Aging Systems In Vitro
8 The Chronological Life Span of Saccharomyces cerevisiae
9 Caloric Restriction and Life Span Determination of Yeast Cells
10 Methods of Nutrigenomics and Longevity Studies in Drosophila: Effects of Diets High in Sucrose， Palmitic Acid， Soy， or Beef
11 Caloric Restriction Mimetics: A Novel Approach for Biogerontology
12 Extension of Cell Life Span Using Exogenous Telomerase
13 Inducing Cellular Senescence Using Defined Genetic Elements
14 Methods of Induction of Cellular Senescence Using Oxidative Stress
15 Nuclear Transfer Methods to Study Aging
16 Use of Ribozymes in Cellular Aging Research
17 Methods of Evaluating the Effect of Pharmacological Drugs on Aging and Life Span in Mice
18 Identifying Genes That Extend Life Span Using a High-Throughput Screening System
19 Application of DNA Microarray Technology to Gerontological Studies
20 Detection and Analysis of Somatic Mutations at a lacZ Reporter Locus in Higher Organisms: Application to Mus musculus and Drosophila melanogaster
21 Subtractive Hybridization Techniques to Study Cellular Senescence
22 Mapping Genetic Polymorphisms Affecting Natural Variation in Drosophila Longevity
23 Quantitative Trait Locus (QTL) Mapping in Aging Systems
24 2D Gel Proteomics: An Approach to Study Age-Related Differences in Protein Abundance or Isoform Complexity in Biological Samples
25 Metabolomics in the Study of Aging and Caloric Restriction
