Protein phosphatase protocols / edited by Greg Moorhead. — Totowa, N.J. : Humana Press, c2007.—(58.17/M592/v.365)

Contents

    Contents
    
    Preface v
    Contributors xi
    1 Analysis of Protein Phosphatases: Toolbox For Unraveling Cell Signaling Networks
    2 A Brief Introduction to the Protein Phosphatase Families
    3 Small-Molecule Inhibitors of Ser/Thr Protein Phosphatases: Specificity， Use， and Common Forms of Abuse
    4 Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin-Sepharose and Microcystin-Biotin-Sepharose
    5 Utilizing Protein Phosphatase Inhibitors to Define PP2A as a Regulator of Ataxia-Telangiectasia Mutated (ATM)
    6 An Automated Fluorescence-Based Method for Continuous Assay of PP2A Activity
    7 An In Vivo Assay to Quantify Stable Protein Phosphatase 2A (PP2A) Heterotrimeric Species
    8 Mutagenesis and Expression of the Scaffolding Aα and Aβ Subunits of PP2A: Assays for Measuring Defects in Binding of Cancer-Related Aα and Aβ Mutants to the Regulatory B and Catalytic C Subunits
    9 Isolation and Characterization of PP2A Holoenzymes Containing FLAG-Tagged B Subunits
    10 Purification of PP2A Holoenzymes by Sequential Immunoprecipitation with Anti-Peptide Antibodies
    11 Purification of PP2Ac from Bovine Heart
    12 Visualization of Intracellular PP1 Targeting Through Transiently and Stably Expressed Fluorescent Protein Fusions
    13 Yeast Two-Hybrid Screens To Identify Drosophila PP1-Binding Proteins
    14 Identification of Cellular Protein Phosphatase-1 Regulators
    15 Assay for Three-Way Interaction of Protein Phosphatase-I (Glc7) with Regulatory Subunits Plus Phosphatase Inhibitor-2
    16 Phosphorylation of the Protein Phosphatase Type 1 Inhibitor Protein CPI-17 by Protein Kinase C
    17 Purification of Smooth Muscle Myosin Phosphatase Using a Thiophosphorylated Myosin Light-Chain-Affinity Resin
    18 Proteins Interacting with Saccharomyces cerevisiae Type 1 Protein Phosphatase Catalytic Subunit Identified by Single-Step Affinity Purification and Mass Spectrometry
    19 Expression of Protein Histidine Phosphatase in Escherichia coli， Purification， and Determination of Enzyme Activity
    20 The Use of RNA Interference to Analyze Protein Phosphatase Function In Mammalian Cells
    21 Recognition of a PP2C Interaction Motif in Several Plant Protein Kinases
    22 Use of Yeast Genetic Tools to Define Biological Roles of Novel Protein Phosphatases
    23 Targeting of PP2C in Budding Yeast
    24 Phosphatase Targets in TOR Signaling
    25 Functional Characterization of the Small CTD Phosphatases
    26 Genome-Scale Discovery and Characterization of Class-Specific Protein Sequences: An Example Using the Protein Phosphatases of Arabidopsis thaliana
    27 Yeast Substrate-Trapping System for Isolating Substrates of Protein Tyrosine Phosphatases
    Index