Baculovirus and insect cell expression protocols / edited by David W. Murhammer. — 2nd ed. — Totowa, N.J. : Humana Press, c2007.—(58.17/M592/v.388)

Contents

    Contents
    
    Preface v
    Contributors xiii
    PART I. INTRODUCTION
    1 Useful Tips， Widely Used Techniques， and Quantifying Cell Metabolic Behavior
    PART II. BACULOVIRUS MOLECULAR BIOLOGY/DEVELOPMENT OF RECOMBINANT BACULOVIRUSES
    2 Introduction to Baculovirus Molecular Biology
    3 Baculovirus Transfer Vectors
    4 Recombinant Baculovirus Isolation
    5 Gene Expression in Mammalian Cells Using BacMam， a Modified Baculovirus System
    Part III. INSECt CELL CULTURE
    6 Available Lepidopteran Insect Cell Lines
    7 Lepidopteran Insect Cell Line Isolation from Insect Tissue
    8 Development of Serum-Free Media for Lepidopteran Insect Cell Lines
    9 Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses
    PART IV. PROTEIN PRODUCTION WITH RECOMBINANT BACULOVIRUSES
    10 Small-Scale Protein Production with the Baculovirus Expression Vector System
    11 Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System
    12 Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors
    13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae
    14 Virus-like Particle and Viral Vector Production Using the B EVS/I nsect Cell System: Adeno-Associated Virus-Based Products
    PART V. RECOMBINANT PROTEIN PRODUCTION WITH TRANSFORMED INSECT CELLS
    15 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression
    16 Transformation of Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression
    17 Transforming Lepidopteran Insect Cells for Improved Protein Processing
    PART Vl. BACULOVIRUS DEVELOPMENT AND PRODUCTION FOR USE AS INSECTICIDES
    18 Introduction to the Use of Baculoviruses as Biological Insecticides
    19 Baculovirus Insecticide Production in Insect Larvae
    20 Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses
    PART VII. MISCELLANEOUS TECHNIQUES AND APPLICATIONS OF THE BACULOVIRUS/INSECT CELL SYSTEM
    21 Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy
    22 Alternative Bioreactor Strategy for Probing Infection and Production
    23 Methods for Gene Silencing with RNAi
    24 Use of the Baculovirus/Insect Cell System to Study Apoptosis
    25 Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells
    Index 461