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Human stem cell manual : a laboratory guide / edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz. — Amsterdam : Elsevier, c2007.—(58.156073/H918)

Contents

    Contents
    
    Part I Basic Methods in Stem Cell Culture 1
    1 Human Embryonic Stem Cell Culture 3
    2 Human Feeder Cells, Feeder-free, and Defined Culture Systems 18
    3 Mouse Embryonic Fibroblast Feeder Cells 34
    4 Cryopreservation of Human Embryonic Stem Cells 47
    Part II Characterization of Stem Cells 57
    5 Classical Cytogenetics: Karyotyping 59
    6 Spectral Karyotyping and Fluorescent in situ Hybridization 71
    7 Genotype and Epigenotype by Single Nucleotide Polymorphism (SNP) Analysis 85
    8 Flow Cytometric Analysis of Human Embryonic Stem Cells 96
    9 Immunocytochemical Analysis of Stem Cells 108
    10 Characterization of Stern Cells Using Reverse Transcriptase 127
    11 Gene Expression Profiling of Stem Cells by Microarray 149
    12 Generation of Human Embryonic Stem Cell-derived Teratomas 162
    13 Characterization of Human Embryonic Stem Cell-derived Teratomas 171
    Part III Differentiation of Human Embryonic Cells 183
    14 Embryoid Bodies and Neuroepithelial Development 185
    15 Motor Neuron and Dopamine Neuron Differentiation 199
    16 Oligodendrocyte Differentiation from Human Embryonic Stem Cells 210
    17 Cardiac Development of Human Embryonic Stem Cells 227
    18 Hematopoiesis from Human Embryonic Stem Cells 238
    Part IV Genetic Manipulation of Stem Cells 253
    19 Genetic Manipulation of Human Embryonic Stem Cells: Lentivirus Vectors 255
    20 Genetic Manipulation of Embryonic Stem Cells 267
    Part V Advanced Methods 289
    21 Derivation of Embryonic Stern Cells from Human Blastocysts 291
    22 Neural Progenitor Cell Culture 309
    23 Stem Cell Transplantation in the Brain 332
    24 In Vitro Fertilization 351
    25 Functional Characterization of Neurons 373
    Part VI Setting up a Laboratory 387
    26 Setting up a Facility for Human Embryonic Stem Cell Research 389
    Part VII Stem Cells and Society 415
    27 Intellectual Property: Owning the Stem Cell 417
    28 Ethical Concerns for Stem Cell Research 426
    29 Guidelines for Embryonic Stem Cell Research Oversight (ESCRO) Committees 437
    Abbreviations 449
    Index 453