Human stem cell manual : a laboratory guide / edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz. — Amsterdam : Elsevier, c2007.—(58.156073/H918) |
Contents
Contents
Part I Basic Methods in Stem Cell Culture 1
1 Human Embryonic Stem Cell Culture 3
2 Human Feeder Cells, Feeder-free, and Defined Culture Systems 18
3 Mouse Embryonic Fibroblast Feeder Cells 34
4 Cryopreservation of Human Embryonic Stem Cells 47
Part II Characterization of Stem Cells 57
5 Classical Cytogenetics: Karyotyping 59
6 Spectral Karyotyping and Fluorescent in situ Hybridization 71
7 Genotype and Epigenotype by Single Nucleotide Polymorphism (SNP) Analysis 85
8 Flow Cytometric Analysis of Human Embryonic Stem Cells 96
9 Immunocytochemical Analysis of Stem Cells 108
10 Characterization of Stern Cells Using Reverse Transcriptase 127
11 Gene Expression Profiling of Stem Cells by Microarray 149
12 Generation of Human Embryonic Stem Cell-derived Teratomas 162
13 Characterization of Human Embryonic Stem Cell-derived Teratomas 171
Part III Differentiation of Human Embryonic Cells 183
14 Embryoid Bodies and Neuroepithelial Development 185
15 Motor Neuron and Dopamine Neuron Differentiation 199
16 Oligodendrocyte Differentiation from Human Embryonic Stem Cells 210
17 Cardiac Development of Human Embryonic Stem Cells 227
18 Hematopoiesis from Human Embryonic Stem Cells 238
Part IV Genetic Manipulation of Stem Cells 253
19 Genetic Manipulation of Human Embryonic Stem Cells: Lentivirus Vectors 255
20 Genetic Manipulation of Embryonic Stem Cells 267
Part V Advanced Methods 289
21 Derivation of Embryonic Stern Cells from Human Blastocysts 291
22 Neural Progenitor Cell Culture 309
23 Stem Cell Transplantation in the Brain 332
24 In Vitro Fertilization 351
25 Functional Characterization of Neurons 373
Part VI Setting up a Laboratory 387
26 Setting up a Facility for Human Embryonic Stem Cell Research 389
Part VII Stem Cells and Society 415
27 Intellectual Property: Owning the Stem Cell 417
28 Ethical Concerns for Stem Cell Research 426
29 Guidelines for Embryonic Stem Cell Research Oversight (ESCRO) Committees 437
Abbreviations 449
Index 453