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Immunodiagnostics : a practical approach / edited by Raymond Edwards. — Oxford : Oxford University Press, 1999.—(63.2445/I33b) |
Contents
Contents
1. Principles of immunodiagnostic tests and their development; with specific use of radioisotopes as tracers 1
1. Introduction
2. Classification of type
3. Selection and preparation of reagents
4. Assay design
2. Solid-phase supports 51
1. Introduction
2. Solid phase supports
3. Preparation of solid supports
4. Passive adsorption
5. Covalent coupling
6. Indirect coupling methods
7. Blocking
8. Storage
9. Assay wash solutions
3. Enzyme-labelled tests with colorimetric， fluorimetric and chemiluminescent detection systems 101
1. Introduction
2. Selection of enzyme-labels
3. Enzyme preparation and assignment of enzymatic activity
4. Conjugation methods
5. Purification， storage and testing of enzyme conjugates
6. Signal generation
4. Time-resolved fluorescence immunoassay 137
1. Introduction
2. Principles of time-resolved fluorescence
3. Protein labelling with lanthanide chelates
4. Storage of labelled proteins
5. Application to two-site immunometric assay of proteins
6. Application to competitive binding immunoassays of haptens
7. Europium-labelled hapten assay
8. Monoclonal antibody screening procedures
9. Novel research assays
10. Non-separation time-resolved fluoroimmunoassay
11. Simultaneous immunoassays
12. Conclusion
5. Light scattering techniques 159
1.Introduction
2. Fundamental aspects of immunoaggregation
3. Non-enhanced methodology
4. Enhanced methodology
5. Summary
6. Enzyme amplification: A means to develop fast ultrasensitive immunoassays 197
1. Introduction
2. Practical applications
3. Fluorimetric enzyme amplification
4. Conclusions
7. Equipment and automation; Appendix on dose-response curve fitting 215
1. Introduction
2. Steps in immunoassay analysis
3. Equipment
4. Analytical considerations
5. Conclusions
8. Quality assurance in immunodiagnostics 243
1.Introduction
2. Quality systems
3. Quality assurance
4. Internal quality control
5. External quality assessment
6. Materials for IQC and EQA
7. Discussion
