Adhesion protein protocols / edited by Amanda S. Coutts. — 2nd ed. — Totowa, N.J. : Humana press, c2007. -- (58.17/M592/v.370)

Contents

    Contents
    
    Preface
    Contributors
    1 Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells
    2 Separation of Cell-Cell Adhesion Complexes By Differential Centrifugation
    3 Analysis of Neutrophil Chemotaxis
    4 Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells
    5 Biochemical Purification of Pseudopodia From Migratory Cells
    6 Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture
    7 Quantitative Analyses of Cell Adhesion Strength
    8 Using RNA Interference to Knock Down the Adhesion Protein TES
    9 Assaying Calpain Activity
    10 Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray
    11 Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells
    12 Bioinformatic Analysis of Adhesion Proteins
    13 Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching
    14 Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture
    15 In Vitro Actin Assembly Assays and Purification From Acanthamoeba
    Index