Cardiac gene expression : Methods and protocols / edited by Jun Zhang, Gregg Rokosh. — Totowa, N.J. : Humana Press, c2007. – (58.17/M592/v.366) |
Contents
Contents
Preface v
Contributors ix
PART I. CARDIAC GENE EXPRESSION: THE GLOBAL PERSPECTIVE
1 Microarray Analysis of Gene Expression in Murine Cardiac Graft Infiltrating Cells
2 Expression Profiling Using Affymetrix GeneChip Probe Arrays
3 Serial Analysis of Gene Expression (SAGE): A Useful Tool to Analyze the Cardiac Transcriptome
4 Functional Genomics by cDNA Subtractive Hybridization
5 Statistical Methods in Cardiac Gene Expression Profiling: From Image to Function
PART II. CARDIAC GENE REGULATION: GENE-SPECIFIC MRNA MEASUREMENT IN THE MYOCARDIUM
6 Measurement of Cardiac Gene Expression by Reverse Transcription Polymerase Chain Reaction (RT-PCR)
7 Quantitative (Real-Time) RT-PCR in Cardiovascular Research
8 RNase Protection Assay for Quantifying Gene Expression Levels
9 In Situ Hybridization: A Technique to Study Localization of Cardiac Gene Expression
Part III. Cardiac GENE Regulation: PROMOTER CHARACTERIZATION IN THE MYOCARDIUM
10 Characterization of cis-Regulatory Elements and Transcription Factor Binding: Gel Mobility Shift Assay
11 Mapping Transcriptional Start Sites and In Silico DNA Footprinting
12 Characterization of Cardiac Gene Promoter Activity: Reporter Constructs and Heterologous Promoter Studies
Part IV. IN Silico Assessment OE REGULATORY cls-ELEMENTS AND GENE REGULATION
13 Comparative Genomics: A Tool to Functionally Annotate Human DNA
14 Developing Computational Resources in Cardiac Gene Expression
PART v. CARDIAC SINGLE NUCLEOTIDE POLYMORPHISMS
15 In Silico Analysis of SNPs and Other High-Throughput Data
16 Discovery and Identification of Sequence Polymorphisms and Mutations with MALDI-TOF MS
Part VI. GENE Overexpression AND Targeting IN the Myocardium
17 Conditional Targeting: Inducible Deletion by Cre Recombinase
18 Cardiomyocyte Preparation, Culture, and Gene Transfer
19 Adeno-Associated Viral Vector-Delivered Hypoxia-lnducible Gene Expression in lschemic Hearts
20 Lentivirus-Mediated Gene Expression