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新书资源(2007年8月)

Cardiac gene expression : Methods and protocols / edited by Jun Zhang, Gregg Rokosh. — Totowa, N.J. : Humana Press, c2007. – (58.17/M592/v.366)

Contents

    Contents
    
    Preface v
    Contributors ix
    PART I. CARDIAC GENE EXPRESSION: THE GLOBAL PERSPECTIVE
    1 Microarray Analysis of Gene Expression in Murine Cardiac Graft Infiltrating Cells
    2 Expression Profiling Using Affymetrix GeneChip Probe Arrays
    3 Serial Analysis of Gene Expression (SAGE): A Useful Tool to Analyze the Cardiac Transcriptome
    4 Functional Genomics by cDNA Subtractive Hybridization
    5 Statistical Methods in Cardiac Gene Expression Profiling: From Image to Function
    PART II. CARDIAC GENE REGULATION: GENE-SPECIFIC MRNA MEASUREMENT IN THE MYOCARDIUM
    6 Measurement of Cardiac Gene Expression by Reverse Transcription Polymerase Chain Reaction (RT-PCR)
    7 Quantitative (Real-Time) RT-PCR in Cardiovascular Research
    8 RNase Protection Assay for Quantifying Gene Expression Levels
    9 In Situ Hybridization: A Technique to Study Localization of Cardiac Gene Expression
    Part III. Cardiac GENE Regulation: PROMOTER CHARACTERIZATION IN THE MYOCARDIUM
    10 Characterization of cis-Regulatory Elements and Transcription Factor Binding: Gel Mobility Shift Assay
    11 Mapping Transcriptional Start Sites and In Silico DNA Footprinting
    12 Characterization of Cardiac Gene Promoter Activity: Reporter Constructs and Heterologous Promoter Studies
    Part IV. IN Silico Assessment OE REGULATORY cls-ELEMENTS AND GENE REGULATION
    13 Comparative Genomics: A Tool to Functionally Annotate Human DNA
    14 Developing Computational Resources in Cardiac Gene Expression
    PART v. CARDIAC SINGLE NUCLEOTIDE POLYMORPHISMS
    15 In Silico Analysis of SNPs and Other High-Throughput Data
    16 Discovery and Identification of Sequence Polymorphisms and Mutations with MALDI-TOF MS
    Part VI. GENE Overexpression AND Targeting IN the Myocardium
    17 Conditional Targeting: Inducible Deletion by Cre Recombinase
    18 Cardiomyocyte Preparation, Culture, and Gene Transfer
    19 Adeno-Associated Viral Vector-Delivered Hypoxia-lnducible Gene Expression in lschemic Hearts
    20 Lentivirus-Mediated Gene Expression