RNA methodologies : a laboratory guide for isolation and characterization = RNA研究方法 : 分离鉴定RNA的实验指导 / Robert E. Farrell, Jr. — 3rd ed.,[reprinted ed.]. — Beijing :Amsterdam : Science Press ; Elsevier Academic Press, 2007. – (58.174251/F245(R)/3rd ed.) |
Contents
Contents
CHAPTER 1 RNA and the Cellular Biochemistry Revisited
CHAPTER 2 Transcription and the Organization of Eukaryotic Genes
CHAPTER 3 Messenger RNA
CHAPTER 4 Resilient Ribonucleases
CHAPTER 5 RNA Isolation Strategies
CHAPTER 6 The Truth About Tissues
CHAPTER 7 Isolation of Polyadenylated RNA
CHAPTER 8 Quality Control for RNA Preparations
CHAPTER 9 Dot Blot Analysis
CHAPTER 10 Electrophoresis of RNA
CHAPTER 11 Photodocumentation and Image Analysis
CHAPTER 12 Northern Analysis
CHAPTER 13 Nucleic Acid Probe Technology
CHAPTER 14 Practical nucleic acid hybridization
CHAPTER 15 Principles of Detection
CHAPTER 16 Quantification of Specific mRNAs by Nuclease Protection
CHAPTER 17 Analysis of Nuclear RNA
CHAPTER 18 cDNA Synthesis
CHAPTER 19 RT-PCR
CHAPTER 20 Quantitative PCR Techniques
CHAPTER 21 Transcript subtraction methods
CHAPTER 22 mRNA Differential Display
CHAPTER 23 High-Throughput Analysis of Gene Expression
CHAPTER 24 RNA Interference: Targeted Gene Silencing
CHAPTER 25 Genomes, Transcriptomes, Proteomes, and Bioinformatics
CHAPTER 26 An RNA Paradigm