Protein biochemistry and proteomics = 蛋白质生物化学与蛋白质组学 / Hubert Rehm. — [Reprinted ed.]. — Beijing : [Amsterdam] : Science Press ; Academic Press, 2007. – (58.17421/R345(R))

Contents

    Contents
    Preface
    Preface to the Fourth Edition
    Acknowledgments
    Abbreviations
    1 Daily Bread
    1.1 Making Buffers
    1.2 Protein Determination
    1.3 Gels
    1.4 Staining Gels
    1.5 Precipitation and Concentration
    1.6 Blotting
    1.7 Autoradiography of Gels and Blots
    2 Ligand Binding
    2.1 Radioactive Ligand Marking
    2.2 Binding
    2.3 Analysis of Binding Data
    2.4 Cross-linking of Ligands
    2.5 Purposes
    3 Solubilization of Membrane Proteins
    3.1 Detergents
    3.2 Solubilization
    4 Protein Detection via Functional Measurements
    4.1 Translocators
    4.2 Reconstitution
    4.3 Flux Assay
    4.4 Constructive Thoughts
    5 Cleaning and Purifying
    5.1 Pure Fun
    5.2 Conventional Purification Methods
    5.3 Affinity Chromatography
    5.4 The Purity Test
    5.5 Profiting
    6 Antibodies
    6.1 Production of Polyclonal Antibodies
    6.2 Immunoprecipitation
    6.3 Immunoaffinity Chromatography
    6.4 Antibodies Against Unpurified Proteins
    6.5 Immunological Detection Techniques
    7 Proteomics
    7.1 Introduction
    7.2 Sample Taking
    7.3 2D Gel Electrophoresis
    7.4 Mass Spectroscopy of Peptides and Proteins
    7.5 Protein Chips
    7.6 Microsequencing
    7.7 Strategy
    8 Subunits
    8.1 Number and Stoichiometry of Subunits
    8.2 What Holds Our World Together?
    9 Glycoproteins
    9.1 How， Where， and for What Purpose Are Proteins Glycosylated?
    9.2 Detecting Glycoproteins in Gels
    9.3 Detection of Glycoproteins on Blots
    9.4 Deglycosylation
    9.5 The Sugar Chains
    10 Treasure Island: Writing Papers
    10.1 Of the Paper
    10.2 Of Writing a Paper
    11 Desert Planet: Researching the Literature
     Last Things
    Appendix A Professional Resources
     A.1 Suppliers
     A.2 Suppliers by Product
    Index