Glycomics / edited by Minoru Fukuda. — Amsterdam ; Boston : Elsevier Academic Press, c2006. – (58.17435/C719/v.416)

Contents

    CONTENTS
    
    Section I. Carbohydrate-Protein Interactions-Physical Measurements
    1. Characterization of Specific Donor Binding to αl，4-N-Acetylhexosaminyltransferase EXTL2 Using Isothermal Titration Calorimetry
    2. NMR Analysis of Carbohydrate-Protein Interactions
    3. X-ray Crystal Structure Determination of Mammalian Glycosyltransferases
    Section II. Phage Display Library
    4. Screening of Peptide-Displaying Phage Libraries to Identify Short Peptides Mimicking Carbohydrates
    5. Phage Display-Derived Human Antibodies Against Specific Glycosaminoglycan Epitopes
    Section III. Glycosyltransferase Expression Profiling
    6. Comprehensive Enzymatic Characterization of Glycosyltransferases with a β3GT and β4GT Motif
    7. Autofluorescent Proteins for Monitoring the Intracellular Distribution of Glycosyltransferases
    8. Expression Profiling of Glycosyltransferases and Related Enzymes Using In Situ Hybridization
    9. Expression Profiling of Glycosyltransferases Using RT-PCR
    10. Expression Profiling of Glycosyltransferases and Related Enzymes Using Gene Microarrays
    Section IV. CHO Mutants in Glycan Biosynthesis
    11. Lectin-Resistant CHO Glycosylation Mutants
    12. CHO Glycosylation Mutants: GPI Anchor
    13. CHO Glycosylation Mutants: Proteoglycans
    Section V. Function of Sulfation
    14. Determination of Substrate Specificity of Sulfotransferases and Glycosyltransferases (Proteoglycans)
    15. Measuring the Activities of the Sulfs: Two Novel Heparin/Heparan Sulfate Endosulfatases
    16. Determination of Chemokine-Glycosaminoglycan Interaction Specificity
    17. Identification of Proteoglycan-Binding Proteins
    18. Functions of Glycans Revealed by Gene Inactivation of L-Selectin Ligand Sulfotransferases in Mice
    Section VI. Functions Revealed by Forced Expression or Gene Knockout
    19. Expression of Specific Carbohydrates by Transfection with Carbohydrate Modifying Enzymes
    20. N-Glycan Structure Analysis Using Lectins and an a-Mannosidase Activity Assay
    21. Targeted Disruption of the Gene Encoding Core 1 fll-3-Galactosyltransferase (T-Synthase) Causes Embryonic Lethality and Defective Angiogenesis in Mice
    22. Roles of Mucin-Type O-Glycans Synthesized by Core2βl，6-N-Acetylglucosaminyltransferase
    23. Analysis of Leukocyte Rolling In Vivo and In Vitro
    24. Cell Type-Specific Roles of Carbohydrates in Tumor Metastasis