 |
YAC Protocols / edited by Alasdair MacKenzie. — 2nd ed. — Totowa, New Jersey : Humana Press, c2006. -- (58.17/M592/v.349) |
Contents
Contents
1 Markers， Selection， and Media in Yeast Artificial Chromosome Cloning
2 Construction of Yeast Artificial Chromosome Libraries From Pathogens and Nonmodel Organisms
3 Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization
4 Analysis of Yeast Artificial Chromosome DNA by Restriction Digestion， Southern Blotting Nucleic Acid Hybridization， and Polymerase Chain Reaction
5 Postgenomic Bioinformatic Analysis of Yeast Artificial Chromosome Sequence
6 Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes
7 Modification and Amplification of Yeast Artificial Chromosomes
8 Modifying Yeast Artificial Chromosomes to Generate Cre/LoxP and FLP/FRT Site-Specific Deletions and Inversions
9 Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes
10 A Polymerase Chain Reaction-Mediated Yeast Artificial Chromosome-Splitting Technology for Generating Targeted Yeast Artificial Chromosomes Subclones
11 Genomic Reconstruction by Serial Mitotic Recombination of Yeast Artificial Chromosomes
12 Targeting Vector Construction by Yeast Artificial Chromosome Modification
13 Production of Yeast Artificial Chromosome Transgenic Mice by Pronuclear Injection of One-Cell Embryos
14 Generation of Yeast Artificial Chromosome Transgenic Mice by Intracytoplasmic Sperm Injection
15 Establishment of Cell Lines That Exhibit Correct Ontogenic Stage-Specific Gene Expression Profiles From Tissues of Yeast Artificial Chromosome Transgenic Mice Using Chemically Induced Growth Signals
16 Application of Yeast Artificial Chromosomes in Fluorescence In Situ Hybridization
17 Contribution of Yeast Artificial Chromosome-Based Physical Maps to the Final Assembly of the Trypanosoma cruzi Genome
Index
