C. elegans : methods and applications / edited by Kevin Strange. — Totowa, New Jersey : Humana Press, c2006. -- (58.17/M592/v.351)

Contents

    Contents
    
    1 An Overview of C. elegans Biology
    2 Comparative Genomics in C. elegans， C. briggsae， and Other Caenorhabditis Species
    3 WormBase: Methods for Data Mining and Comparative Genomics
    4 C. elegans Deletion Mutant Screening
    5 Insertional Mutagenesis in C. elegans Using the Drosophila Transposon Mos1 : A Method for the Rapid Identification of Mutated Genes
    6 Single-Nucleotide Polymorphism Mapping
    7 Creation of Transgenic Lines Using Microparticle Bombardment Methods
    8 Construction of Plasmids for RNA Interference and In Vitro Transcription of Double-Stranded RNA
    9 Delivery Methods for RNA Interference in C. elegans
    10 Functional Genomic Approaches in C. elegans
    11 Assays for Toxicity Studies in C. elegans With Bt Crystal Proteins
    12 Fluorescent Reporter Methods
    13 Electrophysiological Analysis of Neuronal and Muscle Function in C. elegans
    14 Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis
    15 Preservation of C. elegans Tissue Via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immunocytochemistry
    16 Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants
    17 Automated Imaging of C. elegans Behavior
    18 Intracellular Ca2+ Imaging in C. elegans
    19 In Vitro Culture of C. elegans Somatic Cells
    20 Techniques for Analysis， Sorting， and Dispensing of C. elegans on the COPASTM Flow-Sorting System
    Index