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Cytochrome P450 protocols / edited by Ian R. Phillips, Elizabeth A. Shephard. — 2nd ed. — Totowa, New Jersey : Humana Press, c2006.—(58.17/M592/v.320) |
Contents
Contents
Preface
Contributors
1 Cytochrome P450 Nomenclature， 2004
2 Spectral Analyses of Cytochromes P450
3 Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in Escherichia coli
4 Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems
5 Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System
6 Cytochrome P450 Reconstitution Systems
7 Catalytic Assays for Human Cytochrome P450: An Introduction
8 Enzymatic Analysis of cDNA-Expressed Human CYP1A1， CYP1A2， and CYP1 B1 With 7-Ethoxyresorufin as Substrate
9 Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation
10 Determination of CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes
11 High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6(z-Hydroxylation
12 Determination of CYP2C9-Catalyzed Diclofenac 4'-Hydroxylation by High-Performance Liquid Chromatography
13 CYP2C19-Mediated (S)-Mephenytoin 4'-Hydroxylation Assayed by High-Performance Liquid Chromatography With Radiometric Detection
14 CYP2D6-Dependent Bufuralol 1 '-Hydroxylation Assayed by Reverse-Phase Ion-Pair High-Performance Liquid Chromatography With Fluorescence Detection
15 Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation
16 Thin-Layer Chromatography Analysis of Human CYP3A-Catalyzed Testosterone 6[3-Hydroxylation
17 Determination of CYP4A11-Catalyzed Lauric Acid 1 2-Hydroxylation by High-Performance Liquid Chromatography With Radiometric Detection
18 An Isocratic High-Performance Liquid Chromatography Assay for CYP7A1 -Catalyzed Cholesterol 70c-Hydroxylation
19 Use of 7-Ethoxycoumarin to Monitor Multiple Enzymes in the Human CYP1， CYP2， and CYP3 Families
20 Benzydamine N-Oxygenation as a Measure of Flavin-Containing Monooxygenase Activity
21 Prochiral Sulfoxidation as a Probe for Flavin-Containing Monooxygenases
22 Targeting Antipeptide Antibodies Toward Cytochrome P450 Enzymes
23 The Human Cytochrome P450 Allele Nomenclature Committee Web Site: Submission Criteria， Procedures， and Objectives
24 enotyping for Cytochrome P450 Polymorphisms
25 Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing
26 Isolation of Rat Hepatocytes
27 Rat Hepatocyte Cultures: Conventional Monolayer and Cocultures With Rat Liver Epithelial Cells
28 Rat Hepatocyte Cultures: Collagen Gel Sandwich and Immobilization Cultures
29 Hepatocytes in Suspension
30 Isolation of Rat Bone Marrow Stem Cells
31 Transfection of Primary Cultures of Rat Hepatocytes
32 Human Hepatocyte Culture
33 Determination of Cellular Localization of the Expression of Flavin-Containing Monooxygenase Genes in Mouse Tissues by In Situ Hybridization
34 Deletion of Genes From the Mouse Genome Using Cre/IoxP Technology
35 Characterization of Targeted Mouse Embryonic Stem Cell Chromosomes: Karyotyping and Fluorescence In Situ Hybridization of Metaphase Spreads
36 Microinjection of Targeted Embryonic Stem Cells and Establishment of Knockout Mouse Lines for Fmo Genes
37 Use of Reporter Genes to Measure Xenobiotic-Mediated Activation of CYP Gene Transcription
Index 355
