Ion channels : methods and protocols / edited by James D. Stockand, Mark S. Shapiro. — Totowa, New Jersey : Humana Press, c2006.—(58.17/M592/v.337)

Contents

    Contents
    
    Preface
    Contributors
    Part I. Methods for Exogenous Expression of Ion Channels in Cells
    1 Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression System
    2 Overexpression of Proteins in Neurons Using Replication-Deficient Virus
    3 Exogenous Expression of Proteins in Neurons Using the Biolistic Particle Delivery System
    Part II. Methods for Studying Channel Structure-Function
    4 Tertiary and Quaternary Structure Formation of Voltage-Gated Potassium Channels
    5 Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using the Xenopus laevis Oocyte Expression System
    6 Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer Analysis
    Part Ill. Methods for Studying Channel Regulation and Physiological Function
    7 Probing the Effects of Phosphoinositides on Ion Channels
    8 Epithelial Sodium Channel in Planar Lipid Bilayers
    9 A Simple In Vivo Method for Assessing Changes of Membrane-Bound Ion Channel Density in Xenopus Oocytes
    10 Preparation of Cortical Brain Slices for Electrophysiological Recording
    11 Juxtacellular Labeling and Chemical Phenotyping of Extracellularly Recorded Neurons In Vivo
    12 Carbon Fiber Amperometry in the Study of Ion Channels and Secretion
    Part IV. Methods for Studying Channelopathies， Genetic Screening， and Molecular Biology
    13 Genetic Screening for Functionality of Bacterial Potassium Channel Mutants Using K+ Uptake-Deficient Escherichia coil
    14 KCNQ1 K~ Channel-Mediated Cardiac Channelopathies
    15 Tissue-Specific Transgenic and Knockout Mice