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An introduction to molecular biotechnology : molecular fundamentals, methods and applications in modern biotechnology / edited by Michael Wink ; translated by Renate FitzRoy...[et al.] — Weinheim : Wiley-VCH , c2006.—(58.2/I61i) |
Contents
Contents
Part I Fundamentals of Cellular and Molecular Biology
1 The Cell as the Basic Unit of Life 3
2 Structure and Function of Cellular Macromolecules 7
3 Structure and Function of a Cell 39
4 Biosynthesis and Function of Macromolecules (DNA， RNA， and Proteins) 77
5 Distributing Proteins in the Cell (Protein Sorting) 109
6 Diversity of Organisms 121
Part Ⅱ Standard Methods in Molecular Biotechnology
7 Isolation and Purification of Proteins 131
8 Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry 151
9 Isolation of DNA and RNA 165
10 Chromatography and Electrophoresis of Nucleic Acids 171
11 Hybridization of Nucleic Acids 175
12 The Use of Enzymes in the Modification of Nucleic Acids 181
13 Polymerase Chain Reaction (PCR) 189
14 DNA Sequencing 197
15 Cloning Procedures 203
16 Expression of Recombinant Proteins 223
17 The Patch Clamp Method 239
18 Cell Cycle Analysis 247
19 Techniques in Microscopy 257
20 Laser Applications 265
Part III Key Topics
21 Genomics 273
22 Functional Genomics 321
23 Protein-Protein and Protein-DNA Interaction 365
24 Bioinformatics 387
25 Drug Research 413
26 Drug Targeting and Prodrugs 437
27 Molecular Diagnostics in Medicine 455
28 Recombinant Antibodies and Phage Display 479
29 Genetically Modified Mice (Transgenic and Knockout) and their Impact on Medicine 511
30 Gene Therapy: Strategies and Vectors 523
31 Modified DNA， PNA， and Applications in Medicine
32 Plant Biotechnology 557
33 Biocatalysis in the Chemical Industry 583
Part IV Biotechnology in Industry
34 Industrial Application (Biotech Industry， Markets and Opportunities) 615
35 Patents in the Pharmaceutical Biotechnology Industry: Legal and Ethical Issues 631
36 Drug Approval in the European Union and United States 647
37 The Emergence of a Biotechnology Industry 659
38 The 101 of Founding a Biotech Company 671
39 Marketing 683
Glossary 689
Subject Index 733
