Nuclear transfer protocols :cell reprogramming and transgenesis /edited by Paul J. Verma, Alan O. Trounson. --Humana Press,2006. –(58.17/M592/v.348) |
Contents
Contents:
1. Origin and progresss of nuclear transfer in nonmammalian animals 3
2. In vitro maturation and embryo production in cattle 35
3. Activation of Oocytes after nuclear transfer 43
4. Culture medium preferences of pre-implantation cloned mouse embryos 59
5. Maintenance of Pregnancy in pigs with limited viable embryos 79
6. Health and neonatal care of Bovine clones 91
7. Optimization of procedures for cloning by somatic cell nuclear transfer in mice 111
8. Production of cloned calves using rocsovitine-treated adult somatic cells as donors 125
9. A double nuclear transfer technique for cloning pigs 135
10. Nuclear transfer in nonhuman primates 151
11. cloning of exotic/endangered species: desert bighorn sheep 169
12. Handmade somatic cell cloning in cattle 183
13. Genetic modification of sheep by nuclear transfer with gene-targeted somatic cells 199
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