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Nuclear transfer protocols :cell reprogramming and transgenesis /edited by Paul J. Verma, Alan O. Trounson. --Humana Press,2006. –(58.17/M592/v.348)

Contents

 Contents:

1.       Origin and progresss of nuclear transfer in nonmammalian animals  3

2.       In vitro maturation and embryo production in cattle  35

3.       Activation of Oocytes after nuclear transfer  43

4.       Culture medium preferences of pre-implantation cloned mouse embryos  59

5.       Maintenance of Pregnancy in pigs with limited viable embryos  79

6.       Health and neonatal care of Bovine clones  91

7.       Optimization of procedures for cloning by somatic cell nuclear transfer in mice  111

8.       Production of cloned calves using rocsovitine-treated adult somatic cells as donors  125

9.       A double nuclear transfer technique for cloning pigs  135

10.   Nuclear transfer in nonhuman primates  151

11.   cloning of exotic/endangered species: desert bighorn sheep  169

12.   Handmade somatic cell cloning in cattle  183

13.   Genetic modification of sheep by nuclear transfer with gene-targeted somatic cells  199

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