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Cell-cell interactions :methods and protocols /edited by Sean P. Colgan. --Humana Press,2006. –(58.17/M592/v.341) |
Contents
1. Blood-brain barrier and cell-cell interactions: methods for establishing in vitro models of the blood-brain barrier and transport measurements 1
2. Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences 17
3. The role of junctional adhesion molecules in interactions between vascular cells 37
4. Cell-cell interactions on solid matrices 51
5. Cell-cell interactions in the kidney: in the kidney: inducible expression of mutant G protein α-subunits in madin-darby canine kidney cells for studies of epithelial cell junction structure and function 61
6. Nucleotide metabolism and cell-cell interactions 73
7. Analysis of mammalian sperm-egg membrane interactions during in vitro fertileization 89
8. Collagen Gel contraction assay 103
9. Methods to assess tissue permeability 111
10. Bacterial-bacterial cell interactions in biofilms: detection of polysaccharide intercellular adhesions by blotting and confocal microscopy 119
11. A biochemical method for tracking cholera toxin transport from plasma membrane to golgi and endoplasmic reticulum 127
12. Isolation and culture of murine heart and lung endothelial cells for in vitro model systems 141
13. Expression cloning of signaling proteins regulated by cell adhesion 155
14. Assays for the calcium sensitiveity of desmosomes 167
15. Tight junctions and cell-cell interactions 185
16. Simultaneous immuneofluorescence detection of cadherin and β-catenin in mouse tissue sections 197
17. In vitro neutrophil transepithelial migration 205
18. The blot rolling assay: a method for identifying adhesion molecules mediating binding under shear conditions 217
19. Cell-cell interaction in the transcellular biosynthesis of Novel ω-3-Derived lipid mediators 227
20. Isolation and analysis of lipid rafts in cell-cell interactions 283
