1.       Total protein extraction with TCA-Acetone  1

2.       Phenol extraction of proteins for proteomic studies of recalcitrant plant tissues  9

3.       Protein extraction from cereal seeds  15

4.       Protein extraction from Xylem and phloem sap  27

5.       Protein extraction from xylem and phloem sap  27

6.       Isolation of chloroplast proteins from arabidopsis thaliana for proteome analysis  43

7.       Isolation and subfractionation of plant mitochondria for proteomic analysis   49

8.       Extraction of nuclear proteins from root meristematic cells  63

9.       Isolation of nuclear proteins  73

10.   Isolation of cell wall proteins from medicago sativa stems  79

11.   Plant plasma membrane protein extraction and solubilization for proteomic analysis  93

12.   Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis  111

13.   Two-dimensional electrophoresis for plant proteomics  121

14.   Visible and fluorescent staining of two-dimensional gels  145

15.   Two-dimensional differential in-Gel electrophoresis(DIGE) of leaf and roots of lycopersicon esculentum  145

16.   Quantitative analysis of 2D gels  175

17.   Multivariate data analysis of proteome data  195

18.   Edman sequencing of plant proteins from 2D gels  211

19.   Peptide mass fingerprinting: identifyication of proteins by maldi-toe  219

20.   Protein identifyication using nano liquid chromatography-tandem mass spectrometry  235

21.   Two-dimensional nanoflow liquid chromatography-tandem mass spectrometry of proteins extracted from rice leaves and roots  249

22.   Separation, Identification, and profiling of membrane proteins by GFC/IEC/SDS-PAGE and MALDI TOF MS  267

23.   The Proticdb database for 2-DE Proteomics  279

24.   Identification of phosphorlated proteins  305

25.   Plant proteomics and glycosylation  317

26.   Blue-native gel electrophoresis for the characterization of protein complexes in plants  343

27.   Electroelution of intact proteins from SDS-PAGE Gels and their subsequent MALDI-TOF MS Analysis  353

28.   Generation of plant protein microarrays and investingation of antigen-antibody interactions  365

29.   Phosphorylation studies using plant protein microarrays  379