Gene mapping, discovery, and expression :methods and protocols /edited by Minou Bina. --Humana Press, 2006. –(58.17/M592/v.338) |
Contents
Contents:
1. Use of Genome Browsers to Locate your Favorite Genes 1
2. Methods for Identifying and Mapping Recent Segmental and Gene Duplications in Eukaryotic Genomes 9
3. Identification and Mapping of Paralogous Genes on a Known Genomic DNA Sequence 21
4. Quantitative DNA Fiber Mapping in Genome Research and Construction of Physical Maps 31
5. Prins for Mapping Single-Copy Genes 59
6. VISTA Family of Computational Tools for Comparative Analysis of DNA Sequences and Whole Genomes 69
7. Computational Prediction of cis-Regulatory Modules from Multispecies Alignments Using Galaxy, Table Brower, and GALA 91
8. Comparative Promoter Analysis in Vertebrate Genomes with the CORG Workbench 105
9. cis-Regulatory Region Analysis Using BEARR 119
10. A Database of 9-Mers from Promoter Regions of Human Protein-Coding Genes 129
11. A Program Toolkit for the Analysis of Regulatory Regions of Genes 135
12. Analysis of Allele-Specific Gene Expression 153
13. Construction of microRNA-Containing Vectors for Expression in Mammalian Cells 167
14. Mining Microarray Data at NCBI’s Gene Expression Omnibus (GEO) 175
15. The Stanford Microarray Database: A User’s Guide 191
16. Detecting Nucleosome Ladders on Unique DNA Sequences in Mouse Liver Nuclei 209
17. DNA Methyltransferase Probing of DNA-Protein Interactions 225
18. Protein Binding Microarrays (PBMs) for Rapid, High-Through Characterization of the Sequence Specificities of DNA Binding proteins 245
19. Quantitative Profiling of Protein-DNA Binding on Microarrays 261
20. Analysis of Protein-DNA Binding by Streptavidin-Agarose Pulldown 281
21. Isolation and Mass Spectrometry of Specific DNA Binding Proteins 291
22. Isolation of Transcription Factor Complexes by In Vivo Biotinylation Tagging and Direct Binding to Streptavidin Beads 305