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In situ hybridization protocols /edited by Ian A. Darby, Tim D. Hewitson. --Humana Press, 2006. –(58.17/M592/v.326) |
Contents
Contents:
1. Treatment of Tissue Sections for In Situ Hybridization 1
2. Preparation of Template DNA and Labeling Techniques 9
3. In Situ Hybridization Using cRNA Probes: Isotopic and Nonisotopic Detection Methods 17
4. Tyramide Signal Amplification for DNA and mRNA in Situ Hybridization 33
5. Expression Analysis of Murine Genes Using In Situ Hybridization With Radioactive and Noradioactively Labeled Rna Probes 61
6. Nonradioactive in Situ Hybridization on Frozen Sections and Whole Mounts 89
7. In Situ Hybridization of Whole-Mount Embryos 103
8. In Situ Detection of Epstein-Barr Virus and Phenotype Determination of EBV-Infected Cells 115
9. PNA-In Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent Detection of Cellular and Viral Proteins 139
10. Electron Microscopy and in Situ Hybridization: Expression of P2Y2 Receptor mRNA in the Cerebellum 151
11. In Situ Hybridization Using Riboprobes on Free-Floating Brain Sections 163
12. An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells 173
13. Identification of Transplanted Human Cells in Animal Tissues 189
14. In Situ Hybridization to Plant Tissues and Chromosomes 203
15. Histochemical Localization of Cell Proliferation Using In Situ Hybridization for Histone mRNA 219
16. Histochemical Localization of Apoptosis With In Situ Labeling of Fragmented DNA 227
17. The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Tissue Sections 235
18. Semiquanti in Situ Hybridization Using Radioactive Probes to Study Gene Expression in Motoneuron Populations 247
19. Quantitative in Situ Hybridization of Tissue Microarrays 255
