Human embryonic stem cell protocols /edited by Kursad Turksen. -- Humana Press, 2006. –(58.17/M592/v.331)

Contents

    1. Human Embryonic Stem Cells: Isolation， Maintenance， and differentiation 1
    
    2. Blastocyst Culture for Deriving Human Embryonic Stem Cells 13
    
    3. Propagation of Human Embryonic Stem Cells on Human Feeder Cells 23
    
    4. Derivation and Maintenance of Human Embryonic Stem Cells 43
    
    5. Isolation and Characterization of Human Embryonic Stem Cells 55
    
    6. Routine Culture and Differentiation of Human Embryonic Stem Cells 77
    
    7. Culture of Human Embryonic Stem Cells on Human and Mouse Feeder Cells 91
    
    8. Maintenance of Human Embryonic Stem Cells in Animal Serum and Feeder Layer-Free Culture Conditions 105
    
    9. Manipulation of Self-Renewal in Human Embryonic Stem Cells Through a Novel Pharmacological GSK-3 Inhibitor 115
    
    10. Derivationg of Human Feeders for Prolonged Support of Human Embryonic Stem Cells 129
    
    11. Transplantation of Human Embryonic Stem Cells to the Chick Embryo 137
    
    12. Derivation and Characterization of Neuronal Precursors and Dopaminergic Neurons From-Human Embryonic Stem Cell in Vitro 153
    
    13. In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells 179
    
    14. Derivation and Characterization of Hematopoietic Cells From Human Embryonic Stem Cells 179
    
    15. Retroviral Transduction of Hematopoietic Progenitors Derived From Human Embryonic Stem Cells 201
    
    16. Genetic Manipulation of Human Embryonic Stem Cells by Transfection 221
    
    17. Designing， Testing and Validating a Microarray for Stem Cell Characterization 241
    
    18. Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells 267
    
    19. Massively Parallel Signature Sequencing 285
    
    20. Confrontation Cultures of Embryonic Stem Cells With Multicellular Tumor Spheroids to Study Tumor-Induced Angiogenesis 313
    
    21. A Novel Experimental Platform for Investigating Cancer Growth and Anti-Cancer Therapy in a Human Tissue Embryonic Stem Cells 329
    
    22. The Analysis of Mitochondria and Mitochondrial DNA in Human Embryonic Stem Cells 347