Arabidopsis protocols /edited by Julio Salinas and Jose J. Sanchez-Serrano. --Humana Press, 2006. -- (58.17/M592/v.323)

Contents

    1.Growth of Plants and Preservation of Seeds 3
    
    2.Prevention and Control of Pests and Diseases 13
    
    3.Growth of Other Species Related to Arabidopsis thaliana 27
    
    4.Grafting 39
    
    5.Synchronization， Transformation， and Cryopreservation of Suspension-Cultured Cells 45
    
    6.Genetic Analysis 65
    
    7.QTL Analysis 79
    
    8. EMS Mutagenesis of Arabidopsis 101
    
    9.Low-Resolution Mapping of Untagged Mutations 105
    
    10.Gene Identification and Cloning by Molecular Marker Mapping 115
    
    11.High-Throughput TILLING for Arabidopsis 127
    
    12.Genotyping and Mapping With High-Density Oligonucleotide Arrays 137
    
    13.Foward Genetic Screening of Insertional Mutants 147
    
    14.PCR-Based Screening for Insertional Mutants 163
    
    15.Cytogenetic Analysis of Arbidopsis 173
    
    16.Using information From Public Arabidopsis Databases to Aid Research 187
    
    17.In Planta Agrobacterium-Mediated Transformation by Vacuum Infiltration 215
    
    18.Transient Expression Assay by Agroinfiltration of Leaves 225
    
    19.Functional Analysis of Transcription Factors by Microparticle Bombardment 231
    
    20.Transfection Assays with protoplasts containing Integrated Reporter Genes 237
    
    21.Chloroplast Genetic Engineering Via Organogenesis or Somatic
    
     Embryogenesis 245
    
    22.β-Glucuronidase as Reporter Gene: advantages and Limitations 263
    
    23.Use of Fluorescent Proteins as Reporters 275
    
    24. Luciferases as Reporter Genes 293
    
    25.Aequorin as a Reporter Gene 307
    
    26.Applications of Chemical-Inducible Expression Systems in Functional Genomics and Biotechnology 329
    
    27.RNA extraction 345
    
    28.Target Preparation for DNA Microarray Hybridization 349
    
    29.Statistical Issues in Microarray Data Analysis 359
    
    30.Preparation and Quality Assesment of RNA From Cell-Specific Samples Obtained by Laser Microdissection 367
    
    31.Protein Isolation and Second-Dimension Electrophoretic Sparation 381
    
    32.Isolation of Nuclear Proteins 393
    
    33.Purification and Fractionation of Membranes for Proteomic Analysis 403
    
    34.Protein Microsequencing 421
    
    35.Peptide-Based Phosphoproteomics With Immobilized Metal lon Chromatography 431
    
    36.Metabolite Profiling in Arabidopsis 439
    
    37.Hormone Profiling in Arabidopsis 449