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Basic cell culture protocols / edited by Cheryl D. Helgason, Cindy L. Miller. --(Methods in molecular biology ; v.290). --2nd ed. --Humana Press,2005. -- (58.17/M592/v.290)

Contents

Contents

1.Culture of primary Adherent Cells and a Continuously Growing Nonadherent Cell Line  1

2.Detection of Mycoplasma Contaminations  13

3.Eradication of Mycoplasma Contaminations  25

4.Authentication of Scientific Human Cell Lines: Easy-to Use DNA  Fingerprinting  35

5.Cytogenetic Analysis of Cell Lines  51

6.Human and Mouse Hematopoietic Colony-Forming Cell Assays  71

7.Isolation and Culture of Murine Macrophages  91

8.Isolation and Culture of Human Macrophages  105

9.Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture  117

10.In Vitro Generation of Lymphocytes From Embryonic Stem Cells  135

11.Hematopoietic Development of Human Embryonic Stem Cells in Culture  149

12.Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aorta-Gonads-Mesonephros Region  163

13.Culture of Human and Mouse Mesenchymal Cells  173

14.Isolation, Purification, and Cultivation of Murine and human Keratinocytes  187

15.Isolation and Culture of Primary Human Hepatocytes  207

16.Primary Kidney Proximal Tubule Cells  231

17.Enzymatic Dissociation and Culture of Normal human Mammary Tissue to Detect Progenitor Activity  249

18.Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System Tissue  265

19.Isolation and Culture of Skeletal Muscle Myfibers as a Means to Analyze Satellite Cells  281

20.Adult Ventricular Cardiomyocytes: Isolation and Culture  305

21.Isolation and Culture of Primary Endothelial Cells  315

22.Studying Leukocyte Rolling and Adhesion in Vitro Under Flow Conditions  331

23.Isolation and Characterization of Side Population Cells  343

24.Scalable Production of Embryonic Stem Cell-Derived Cells  353