Epigenetics Protocols / edited by Trygve O. Tollefsbol. -- (Methods in molecular biology ; v.287). -- Humana Press, 2004. -- (58.17/M592/v.287)

Contents

Contents 1. Methods of Epigenetic Analysis  1  2.Chromatin Immunoprecipitation Assays  9 3.Native Chromatin Immunoprecipitation  21 4.Q-PCR in Combination With ChIP Assays to Detect Changes in Chromatin Acetylation  45 5.Restriction Endonuclease Accessibility as a Determinant of Altered chromatin Structure  53 6.Measuring Changes in Chromatin Using Micrococcal Nuclease  657.DNasel Hypersensitivity Analysis of chromatin Structure  77 8.Inhibition of Histone Deacetylases  87 9.Site-Specific Analysis of Histone Methylation and Acetylation  99. 10.Analysis of Mammalian Telomere Position Effect  121 11.Activity Assays for Poly-ADP Ribose Polymerase 1  137 12Multigenerational Selection and Detection of Altered Histone Acetylation and Methylation Patterns: Toward a Quantitative Epigenetics in Drosophila  151 13.Profiling DNA Methylation by Bisulfite Genomic Sequencing: Problems and Solutions  169 14.Methylation-Sensitive Single-Starand Conformation Analysis: A Rapid Method to Screen for and Analyze DNA Methylation  181 15.SIRPH Analysis: SNuPE With IP-RP-HPLC for Quantitative Measurements of DNA Methylation at Specific CpG Sites  195 16.Real-Time PCR-Based Assay for Quantitative Determination of Methylation Status  207 17.Denaturing Gradient Gel Electrophoresis to Detect Methylation Changes in DNA  219 18.Photocrosslinking Oligonucleotide Hybridization Assay for Concurrent Gene Dosage and CpG Methylation Analysis  233 19.Methylation-Specific Oligonucleotide Microarray  251 20.Methylation-Specific PCR in Situ Hybridization  261 21.Relative Quantitation of DNA Methyltransferase mRNA by Real-Time RT-PCR Assay  273 22.DMB (DNMT-Magnetic Beads) assay: Measuring DNA Methyltransferase Activity in Vitro  285