Oligonucleotide synthesis : methods and applications / edited by Piet Herdewijin. -- (Methods in molecular biology ; v. 288). -- Humana Press, 2005. -- (58.17/M592/v.288)

Contents:

1. Synthesis of DNA Using a New Two-Step Cycle  1

2. RNA Synthesis Using 2'-O-(Tert-Butyldimethylsilyl) Protection  17

3. RNA Oligonucleotide Synthesis Via 5'-Sily-2'-Orthoester Chemistry  33

4. Dimethylthiarum Disulfide: New Sulfur Transfer Reagent in Phosphorothioates Oligonucleotide Synthesis  51

5. Assay for Evaluating Ribonuclease H-Mediated Degraqdation of RNA-Antisense Oligonucleotide Duplexes  65

6. Di- and Oligonucleotide Synthesis  81

7. Solid-Phase Synthesis of Circular Oligonucleotide  101

8. Locked Nucleic Acid Synthesis  127

9. Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-pligomers  147

10. Base-Modified Oligonucleotides With Increased Duplex Stability: Pyrazolo[3,4-dlpyrimidines Replacing Purines  165

11. Introduction of Hypermodified Necleotides in RNA  187

12. Chemical Methods for Peptode-Oligonucleotide Conjugate Synthesis  187

13. Biotin-Labeled Oligonucleotides With Extraordinarily Long Tethering Arms  225

14. Universal Labeling Chemistry for Nucleic Acid Detection on DNA Chips  24115. Poatsynthetic Functionalization of Triple Helix-Forming Oligonucleotide  241

15. Postsynthetic functionalization of Triple Helix-Forming Oligonucleotide  251

16. Fluorescence-Based on-Line Detection as an Analytical Tool in RNA Electrophoresis  261

17. Design and Optimization of Molecular Beacon Real-time Polymerase Chain Reaction Assays  273

18. High-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct Sequencing  291

19. Nonenzymatic Template-Directed RNA synthesis  305

20. DNAse I footprinting of Small Molecule binding Sites on DNA  319

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