Checkpoint controls and cancer. v.2: Activation and regulation protocols / edited by Axel H. Sch"othal. -- (Methods in molecular biology ; v. 281). -- Humana Press, 2004. -- (58.17/M592/v.281)

Contents:

1. Analysis of RB Action in DNA Damage Checkpoint Response  3

2. Interaction Between the Retinoblastoma Protein and Protein Phosphatase 1 During the Cell Cycle  17

3. Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-binding Site Analysis  33

4. Functional Analysis of CDK Inhibitor p21 (WAF1)  55

5. Analysis of p21(CDKN1A) Recruitment to DNA Excision Repair Foci in the UV-Induced DNA Damage Response  73

6. Quantitative Determination of p16 Gene Expression by RT-PCR  91

7. Measuring Cyclin-Dependent Kinase Activity  105

8. Determination of the Catalytic Activities of mTOR and Other  Members of the Phosphoinositide-3-Kinase-Related Kinase Family  125

9. CHK1 Kinase Activity Assay  143

10. Assaying Cdc25 Phosphatase Activity  153

11. Analyzing the Regulation and Function of ATM  163

12. Use of siRNA to Study the Function of MDC1 in DNA Damage Responses  179

13. Functional Analysis of APC-Cdh1  189

14. Purification of Mitotic Checkpoint Complex, an Inhibitor of the APC/C From Hela Cells  199

15. Analysis of the Spindle-Assembly Checkpoint in Hela Cells  213

16. Functional Analysis of the Spindle-Checkpoint Proteins Using an In Vitro Ubiquitination Assay  227

17. Analysis of Checkpoint Responses to Histone Deacetylase Inhibitors  245

18. Biochemical analysis of the Cell Cycle and Cell Cycle Checkpoints in Transiently Transfected Cells After Collection With Magnetic Beads  261

19. Analysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA Substrates  271

20. Analyzing Cell Cycle Checkpoints After Ionizing Radiation  283

21. FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells  293

22. Analysis of Cell Cycle By Flow Cytometry  301

23. Analyzing Markers of Apoptosis In Vitro  313

24. Analysis of Telomerase Activity and Telomere Function in Cancer  333