Checkpoint controls and cancer. v.1: Reviews and model systems / edited by Axel H. Sch"othal. -- (Methods in molecular biology ; v. 280). -- Humana Press, 2004. -- (58.17/M592/v.280)

Contents

Contents:

1. G1 and S-Phase Checkpoints, Chromosome Instability, and Cancer  3

2. Analyzing the G2/M Checkpoint  51

3. Analyzing the Spindle Checkpoint in Yeast and Frogs  83

4. Cell Cycle Checkpoint Control Mechanisms That Can Be Disrupted in Cancer  99

5. Establishment of a Cell-Free System to Study the Activation of chk2  165

6. Analyzing Checkpoint controls in Human Skin  175

7. Generation and analysis of Brca1 Conditional Knockout Mice  185

8. Analysis of Cell Cycle Progression and Genomic Integrity in Early Lethal Knockouts  201

9. Xenopus Cell-Free Extracts to Study the DNA Damage Response  213

10. A Xenopus Cell-Free System for Functional analysis of the Chfr Ubiquitin Ligase Involved in Control of Mitotic Entry  229

11. Control of Mitotic Entry After DNA Damage in Drosophila  245

12. Methods for Analyzing Checkpoint Responses in Caenorhabditis elegans  257

13. Assaying the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisias  275

14. Purification and analysis of Checkpoint Protein Complexes From Saccharomyces cerevisiae  291