Diabetes mellitus : methods and protocols / edited by Sabire "Ozcan. -- (Methods in molecular medicine ; v. 83). -- Humana Press. -- 63.8511/D536t

Contents:

1. Isolation of Islets of Langerhans from Rodent Pancreas  3

2. Purification of Rat Pancreatic β-Cells by Fluorescence-Activated Cell Sorting  15

3. Assessment of Insulin Secretion in the Mouse  23

4. Detection of Insulin Production by Immunohistochemistry  47

5. Quantification of the Level of Insulin Gene Expression  51

6. Chromatin Immunoprecipitation Using Isolated Islets of Langerhans  61

7. Adenoviral Gene Transfer into β-Cells Lines  73

8. Utilization of NOD Mice in the Study of type 1 Diabetes  81

9. Introduction of DNA into 3T3-L1 Adipocytes by Electroporation  93

10. Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes  97

11. Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes  105

12. Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assay  113

13. Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates  119

14. Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity  127

15. Assaying AKT/Protein Kinase B Activity  137

16. Measurements of Cellular Phosphoinositide Levels in 3T3-L1 Adipocytes  145

17. Measurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 Adipocytes  155

18. Measurement of Contraction-Stimulated GLUT-4 Translocation in Isolated Skeletal Muscle  163

19. Single Embryo Measurement of Basel- and Insulin-Stimulated Glucose Uptake  171

20. Immunohistologic Staining of Muscle and Embryos to Detect Insulin-Stimulated Translocation of glucose Transporters  179