Protein purification protocols / edited by Paul Cutler. -- 2nd ed. -- (Methods in molecular biology ; v.244). -- Humana Press, 2004 |
Contents
1. General Strategies 1
2. Preparation of Extracts from Animal Tissues 15
3. Protein Extraction from Plant Tissues 21
4. Extraction of Recombinant Protein From Bacteria 29
5. Protein Extraction from Fungi 37
6. Subcellular Fraction of Animal Tissues 47
7. Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria 53
8. The Extraction of Enzymes from Plant nTissues Rich in Phenolic Compounds 65
9. Avoidance of Proteolysis in Extracts 75
10. Concentration of Extracts 85
11. Making and Changing Buffers 91
12. Purification and Concentration by Ultrafiltration 101
13. Bulk Purification by Fractional Precipitation 117
14. Ion-Exchange Chromatography 125
15. Hydrophobic Interaction Chromatography 133
16. Affinity Chromatography 139
17. Dye-Ligand Affinity Chromatography 151
18. Lectin Affinity Chromatography 159
19. Immunoaffinity Chromatography 167
20. Immobilized Metal-Ion Affinity Chromatography 179
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