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Protein purification protocols / edited by Paul Cutler. -- 2nd ed. -- (Methods in molecular biology ; v.244). -- Humana Press, 2004

Contents

1. General Strategies  1

2. Preparation of Extracts from Animal Tissues  15

3. Protein Extraction from Plant Tissues  21

4. Extraction of Recombinant Protein From Bacteria  29

5. Protein Extraction from Fungi  37

6. Subcellular Fraction of Animal Tissues  47

7. Subcellular Fractionation of Plant Tissues: Isolation of  Plastids and Mitochondria  53

8. The Extraction of  Enzymes from Plant nTissues Rich in Phenolic Compounds  65

9. Avoidance of  Proteolysis in Extracts  75

10. Concentration of Extracts  85

11. Making and Changing Buffers  91

12. Purification and Concentration by Ultrafiltration  101

13. Bulk Purification by Fractional Precipitation  117

14. Ion-Exchange Chromatography  125

15. Hydrophobic Interaction Chromatography  133

16. Affinity Chromatography  139

17. Dye-Ligand Affinity Chromatography  151 

18. Lectin Affinity Chromatography  159

19. Immunoaffinity Chromatography  167

20. Immobilized Metal-Ion Affinity Chromatography  179

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