Ribozymes and siRNA protocols / edited by Mouldy Sioud. -- 2nd ed. -- (Methods in molecular biology ; v.252). -- Humana Press, 2004 |
Contents
1. Ribozyme- and siRNA-Mediated mRNA Degradation: A General Intoduction 1
2. Combination of Chemical and Enzymatic RNA Synthesis 9
3. Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes 19
4. Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection 33
5. Quantification of Ribozyme Target RNA Using Real-Time PCR 49
6. Analysis of Ribozyme Structure and Function by Nucleotide Analog Interference Mapping 57
7. Analysis of Global Conformational Transitions in Ribozymes 77
8. In Vivo Detection of Ribozyme Cleavage Products and RNA Structure by Use of Terminal Transferase-Dependent PCR 109
9. Identification of Efficient Cleavage Sites in Long-Target RNAs 125
10. Selection In Vitro of Allosteric Ribozymes 145
11. Regulation of Ribozyme Cleavage Activity by Oligonucleotides 165
12. Tetracycline-Regulated Expression of Hammerhead Ribozymes In Viv0 179
13. Ribozyme Expression Systems 195
14. Design and Expression of Chimeric U1/Ribozyme Transgenes 209
15. Design and Validation of Therapeutic Hammerhead Ribozymes for Autosomal Dominant Diseases 221
16. Helicase-Attached Novel Hybrid Ribozymes 237
17. Functional Gene Discovery Using Hybrid Ribozyme Libraries 245
18.Maxizyme Technology 257
19. Target=Site Selection for the 10-23 DNAzyme 267
20. In Vitro Selected RNA-Cleaving DNA Enzymes From Combinatorial Libraries 279
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