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Ribozymes and siRNA protocols / edited by Mouldy Sioud. -- 2nd ed. -- (Methods in molecular biology ; v.252). -- Humana Press, 2004

Contents

1. Ribozyme- and siRNA-Mediated mRNA Degradation: A General Intoduction  1

2. Combination of Chemical and Enzymatic RNA Synthesis  9

3. Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes  19

4. Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection  33

5. Quantification of Ribozyme Target RNA Using Real-Time PCR  49

6. Analysis of Ribozyme Structure and Function by Nucleotide Analog Interference Mapping  57

7. Analysis of Global Conformational Transitions in Ribozymes  77

8. In Vivo Detection of Ribozyme Cleavage Products and RNA Structure by Use of Terminal Transferase-Dependent PCR  109

9. Identification of Efficient Cleavage Sites in Long-Target RNAs  125

10. Selection In Vitro of Allosteric Ribozymes  145

11. Regulation of Ribozyme Cleavage Activity by Oligonucleotides  165

12. Tetracycline-Regulated Expression of Hammerhead Ribozymes In Viv0  179

13. Ribozyme Expression Systems  195

14. Design and Expression of Chimeric U1/Ribozyme Transgenes  209

15. Design and Validation of Therapeutic Hammerhead Ribozymes for Autosomal Dominant Diseases  221

16. Helicase-Attached Novel Hybrid Ribozymes  237

17. Functional Gene Discovery Using Hybrid Ribozyme Libraries  245

18.Maxizyme Technology  257

19. Target=Site Selection for the 10-23 DNAzyme  267

20. In Vitro Selected RNA-Cleaving DNA Enzymes From Combinatorial Libraries  279

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