Parasite Genomics Protocols / edited by Sara E. Melville. -- (Methods in molecular biology ; v. 270). -- Humana Press, 2004

Contents

索书号： 58.17/M592/v.270

1. Sequencing Stratefies for Parasite Genomes  1

2. Annotation of Parasite Genomes  17

3. Parasite Genome Databases and Web-Based Resources  45

4. Expressed Sequence Tags: Medium-Throughput Protocols  75

5. Expressed Sequence Tags: Analysis and Annotation  93

6. Positive Selection Scanning of Parasite DNA Sequences  127

7. RACE and RAGE Cloning in Parasitic Microbial Eukaryotes  151

8. Amplified (Restriction) Fragment Length Polymorphism (AELP) Analysis  173

9. Minisatellites and MVR-PCR for the Individual Indentification of Parasite Isolates  187

10. Analysis of Differentially Expressed Parasite Genes and Proteins Using Transcriptomics and Proteomics  203

11. Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays  219

12. DNA Content Analysis on Microarrays  237

13. Typing Single-Nucleotide Polymorphisms in Toxoplasma gondii by Allele-Specific Primer EXTENSION AND MICROARRAY Detection  249

14. Transfection of the Human Malaria Parasite Plasmodium falciparum  263

15. A PCR-Based Method for Gene Deletion and Protein Tgging in Trypanosoma brucei  277

16. Analysis of Gene function in Trypanosoma brucei Using RNA Interference  287

17. In Vitro Shuttle Mutagenesis Using Engineered Mariner Teansposons  299

18. Random Mutagenesis Strategies for Construction of Large and Diverse Clone Libraries of Mutated DNA Fragments  319

19.Separation, Digestion, and Cloning of Intact Parasite Chromosomes  335

20. Chromosome Fragmentation in Leishmania  353

21.  FISH Mapping for Helminth Genomes  379

22. FISH-FISH: Fluorescence In Situ Hybridization on Stretched DNA  395

23. Yeast Two-Hybrid Assay for Studying Protein-Protein Interactions  403

24. From Genomes to Vaccines for Leishmaniasis  423