Directed Evolution Library Creation : methods and Protocols / edited by Frances H. arnold and George Georgiou. -- (Methods in molecular biology ; v. 231). -- Humana, 2003

Contents

索书号： 58.17/M592/v.231

1. Generating Mutant Libraries using Error-Prone PCR  3

2. Preparing Libraries in Escherichia coli  11

3. Preparing Libraies in Saccharomyces cerevisiae  17

4. Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP)  23

5. Construction of Designed Protein Libraries Using Gene Assembly Mutagenesis  29

6. Production of Randomly Mutated Plasmid Libraries Using Mutator Strains  39

7. Evolution of Microorganisms Using Mutator Plasmids  45

8. Random Insertion and Deletion Mutagenesis  53

9. Random Oligonucleotide Mutagenesis  65

10. Saturation Mutagenesis  75

11. DNA Shuffling  85

12. Family shuffling with Single-Stranded DNA  91

13. In Vitro DNA Recombination by Random Priming  99

14. Staggered Extension Process (SrEP) in Vitro Recombination  105

15. RACHITT: Gene Family shuffling by Random Chimeragenesis  111

16. The Creation of  ITCHY Hybrid Protein Libraries  129

17. Preparation of SCRATCHY Hybrid Protein Libraies: Size- and In-Frame Selection of Nucleic Acid Sequences  143

18. Sequence Homology-Independent Protein Recombination (SHIPREC)  153

19. Producing Chimeric Genes by CLERY : In Vitro and In Vivo Recombination  165

20. Analysis of Shuffled Libraies by Oligonucleotide Probe Hybridization  177

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