Rapid cycle Real-Time PCR - Methods and Applications : quantification / edited by Carl Wittwer ... [et al.]. -- Springer, 2004

Contents

58.174354/R218

Contents:

1. Housekeeping Genes: A Gold Standard?  3

2. The Choice of House Keeping Genes in MRD-Quantification of AML1-ETO Positive Acute Myeloid Leukemia  11

3. Quantification of mRNA Using Linear Regression of Log-Linear PCR Data-Points as an Alternative of the Standard Curve Approach  21

4. Measuring Genome Sizes by Absolute Quantification  31

5. Relative Quantification of Insulin Gene Expression on the LigheCycler Using SYBR Green I  45

6. Quantification of  δRec-ψJα Signal Joint T-Cell Receptor Excision Circle DNA in Patients after Autologous and Allogeneic Stem Cell Transplantation  53

7. Expression Analysis of Mitochondrial Components in a Variety of Plant Species Using Real-Time Quantitative PCR  61

8. Quantification of Ikaros Splice Variants by Real-Time PCR  73

9. Methods to Quantify Cytokine Gene Expression by Real-Time PCR  83

10. Profiling Breast Cancer Using Real-Time Quantitative PCR  95

11. HER-2/neu Gene Copy Number Quantified by Real-Time PCR in Cell Lines and Breast Cancer Tissue  107

12. Quantification of Several Components of Fibrinolytic and Matrix Metalloproteinase Systems in Primary Breast Cancer  117

13. A Rapid Cycle Real-Time Quantitative B-globin PCR for Quantification of Human DNA in Feces  125

14. Quantification of Tumor Load for Follicular Lymphoma by Quantifying BCL2/IGH Using Real-Time Quantitative PCR by LightCycle(TM)  131

... ...