Human retrovirus protocols : virology and molecular biology / edited by Tuofu Zhu. -- (Methods in molecular biology ; v. 304). -- Humana Press, 2005. -- (58. 17/M592/v.304) |
Contents
Contents:
1. Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals 3
2. Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals 17
3. Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes 25
4. Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid 35
5. Isolation and HIV -1 Infection of Primary Microglia From Fetal and Adult Tissue 49
6. Isolation of Human Immunodeficience Virus Type 1 From Semen and Vaginal Fluids 71
7. Isolation and Quantification of HIV From Lymph Nodes 87
8. Isolation and Human Immunodeficiency Virus Type 2 Biological Clones From Peripheral Blood Lymphocytes 95
9. Isolation and Confirmation of Human T-Cell Leukemia Virus Type 2 From Peripheral Blood Mononuclear Cells 113
10. Isolation of Foamy Viruses From Peripheral Blood Lymphocytes 125
11. alu-LTR Real-Time Nested PCR Assay for quantifying Integrated HIV-1 DNA 139
12. Quantification of HFV-Integrated DNA in Human Cells by Alu-LTR Real-Time PCR 155
13. Detection of HIV-1 Gene on Cytologic Smear Slides 183
14. Detection of HTLV-1 Gene Cytologic Smear slides 183
15. Detection of HIV-2 by PCR 191
16. Quantitation of HIV-1 Viral RNA in Blood Plasma amd Genital Secretions 201
17. Quantification of Proviral DNA Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR 215
18. Plasma RNA Viral Load in HIV-1 Group O Infection by Real-Time PCR 221
19. A New combined HIV p24 antigen and Anti-HIV-1/2/O Screening Assay 229
20. A New Automated Forth-Generation HIV Screening Assay with Sensitive Antigen Detection Module and High Specifity 245
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