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Human retrovirus protocols : virology and molecular biology / edited by Tuofu Zhu. -- (Methods in molecular biology ; v. 304). -- Humana Press, 2005. -- (58. 17/M592/v.304)

Contents

Contents:

1. Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals  3

2. Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals  17

3. Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes  25

4. Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid  35

5. Isolation and HIV -1 Infection of Primary Microglia From Fetal and Adult Tissue  49

6. Isolation of Human Immunodeficience Virus Type 1 From Semen and Vaginal Fluids  71

7. Isolation and Quantification of HIV From Lymph Nodes  87

8. Isolation and Human Immunodeficiency Virus Type 2 Biological Clones From Peripheral Blood Lymphocytes  95

9. Isolation and Confirmation of Human T-Cell Leukemia Virus Type 2 From Peripheral Blood Mononuclear Cells  113

10. Isolation of Foamy Viruses From Peripheral Blood Lymphocytes  125

11. alu-LTR Real-Time Nested PCR Assay for quantifying Integrated HIV-1 DNA  139

12. Quantification of HFV-Integrated DNA in Human Cells by Alu-LTR Real-Time PCR  155

13. Detection of HIV-1 Gene on Cytologic Smear Slides  183

14. Detection of HTLV-1 Gene Cytologic Smear slides  183

15. Detection of HIV-2 by PCR  191

16. Quantitation of HIV-1 Viral RNA in Blood Plasma amd Genital Secretions  201

17. Quantification of Proviral DNA Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR  215

18. Plasma RNA Viral Load in HIV-1 Group O Infection by Real-Time PCR  221

19. A New combined HIV p24 antigen and Anti-HIV-1/2/O Screening Assay  229

20. A New Automated Forth-Generation HIV Screening Assay with Sensitive Antigen Detection Module and High Specifity  245

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