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E. coli plasmid vectors : methods and applications / edited by Nicola Casali and Andrew Prston. -- (Methods in molecular biology ; v.235). -- humana Press, 2003

Contents

索书号: 58.17/M592/v.235

1. The Function and Organization of Plasmids  1

2. Choosing a Cloning Vector  19

3. Escherichia coli Host Strains  27

4. Chemical Transformation of E. coli  49

5. Eletroporation of E. coli  55

6. DNA Transfer by Bacterial Conjugation  61

7. Cosmid Packaging and Infection of E. coli  67

8. Isolation of Plasmids from e. coli by Alkaline Lysis  75

9. Isolation of Plasmids from E. coli by Boiling Lysis  79

10. High-Purity Plasmid Isolation Using Silica Oxide  83

11. High-Throughput Plasmid Extraction Using Microtiter Plates  89

12. Isolation of Cosmid and BAC DNA from E. coli.  99

13. Preparation of Single-Stranded DNA from Phagemid Vectors  103

14. Using Desktop Cloning Software to Plan, Track and Evaluate Cloning Projects  107

15. Cloning in Plasmid Vectors  121

16. Extraction of DNA from Agarose Gels  137

17. Cloning PCR Products with T-Vectors  141

18. Construction of Genomic Libraries in λ-Vectors  153

19. Rapid Screening of Recombinant Plasmids  169

20. Restriction Analysis of Recombinant Plasmids  175

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