E. coli plasmid vectors : methods and applications / edited by Nicola Casali and Andrew Prston. -- (Methods in molecular biology ; v.235). -- humana Press, 2003 |
Contents
索书号: 58.17/M592/v.235
1. The Function and Organization of Plasmids 1
2. Choosing a Cloning Vector 19
3. Escherichia coli Host Strains 27
4. Chemical Transformation of E. coli 49
5. Eletroporation of E. coli 55
6. DNA Transfer by Bacterial Conjugation 61
7. Cosmid Packaging and Infection of E. coli 67
8. Isolation of Plasmids from e. coli by Alkaline Lysis 75
9. Isolation of Plasmids from E. coli by Boiling Lysis 79
10. High-Purity Plasmid Isolation Using Silica Oxide 83
11. High-Throughput Plasmid Extraction Using Microtiter Plates 89
12. Isolation of Cosmid and BAC DNA from E. coli. 99
13. Preparation of Single-Stranded DNA from Phagemid Vectors 103
14. Using Desktop Cloning Software to Plan, Track and Evaluate Cloning Projects 107
15. Cloning in Plasmid Vectors 121
16. Extraction of DNA from Agarose Gels 137
17. Cloning PCR Products with T-Vectors 141
18. Construction of Genomic Libraries in λ-Vectors 153
19. Rapid Screening of Recombinant Plasmids 169
20. Restriction Analysis of Recombinant Plasmids 175
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