Bacterial Artificial Chromosomes : v.1. Library construction, physical mapping, and sequencing. -- (Methods in Molecular Biology ; v.255) / edited by Shaying Zhao and Marvin Stodolsky. -- 2004

Contents

索书号：58.17/M592/v.255

Contents:1

1. BAC Library Construction  1

2.Construction of Small Genome BAC Library for Functional  and Genomic Applications  47

3.Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packging  57

4.Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions  69

5.Purification of BAC DNA  91

6.Hybridization-Based Selection of BAC Clones  101

7.Applications of Interspersed Repeat Sequence Polymerase Chain Reaction  113

8.BAC Mapping Using Fluorescence In Situ Hybridization  131

9. High-Throughput BAC Fingerprinting  143

10.BAC End Sequencing  157

11.Radiation Hybrid Mapping With BAC Ends  163

12.Shotgun Library Construction for DNA Sequencing  171

13.Rolling Circle Amplification for Sequencing Templates  189

14.Transposon-Mediated Sequencing  197

15.Pyrosequencing: A Tool for DNA Sequencing Analysis  211

16.Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions  221

17.Retrofitting BACs and PACs with LoxP Transposons to Generate Nested Deletions  231

18.Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers  243

19.BAC Finishing Strategies  255

20.Using the TIGR Assembler in Shotgun Sequencing Projects  279

21.Finishing"Working Draft" BAC Projects by Directed Sequencing With TheroFidelase and Fimers  295

22.Optimized Multiplex Polymerase Chain Reaction: An Effective Method for Rapid Gap Closure  309

23.Assembly of DNA Sequencing Data  319

24.Sequences Finishing  333

25.Quality Assessment  of Finished BAC Sequences  343