Methods in Cell Biology :v.71, Neurons: Methods and Applications for the Cell Biologist / edited by Peter J. Hollenbeck and James R. Bamburg. -- Academic Press, 2003

索书号: 58.1574/M592/V.71

Contents:

1. Comparing the Properties of Neuronal Culture Systems: A Shopping Guide for the Cell Biologist  2

2. Growing and Working with Peripheral Neurons  18

3. Dissection and Culturing of Chick Ciliary Ganglion Neurons: A System Well Suited to Synaptic Study  38

4.The Culture of Chick Forebrain Neurons  52

5.Growing and Working with Spinal Motor Neurons  68

6.Avian Purkinje Neuronal Cultures: Extrinsic Control of Morphology bu Cell Type and Glutamate  90

7.Culturing Hippocampal and Cortical Neurons  112

8. Working with Xenopus Spinal Neurons in Live Cell Culture  130

9.Culturing Neurons from the Snail Helisoma  158

10. The Tibial-1 Pioneer Pathway: An in Vivo Model for Neuronal Outgrowth and Guidance  172

11.Techniques to Dissect Cellular and Subcellular Function in the Drosophila Nervous System  196

12.PC12 Cells as a Model Studies of Regulated Secretion in Neuronal and Endocrine Cells  268

13.B35 Neuroblastoma Cells: An Easily Transfected, Cultured Cell Model of Central Nervous Sytem Neurons  288

14.Live-Cell Imaging of Slow Axonal Transport in Cultured Neurons  306

15.Making Proteins into Drugs: Assisted Delivery of Proteins and Peptides into Living Neurons  326

16.Tranfection of Primary Central and Peripheral  Nervous System Neurons  340

17.Biolistic Transfection  354

18.Expression of Transgenes in Primary Neurons from Chick Peripheral and Central Nervous

Systems by Retroviral Infection of Early Embryos  370

19.Production and Use of Replication-Deficient Adenovirus for Transgene Expression in Neurons  388